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Episode 39: Spatial proteomics at Pixelgen
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Tune in to hear all about Pixelgen Technologies - creators of a novel technology for doing single-cell spatial proteomics.
We chat about how this fascinating technology works, what you can do with the data and why they chose to develop their analysis suite as an open-source nf-core pipeline.
Links:
- Software:
- Science:
- Nature Methods publication: “Molecular pixelation: spatial proteomics of single cells by sequencing”
- Presentations:
- Nextflow Summit 2022 talk: “Pixelgen Technologies ❤︎ Nextflow”
- Nextflow Summit 2024 Boston talk: “Nextflow allows Pixelgen innovation engine to reach the market quicker”
- Nextflow Summit 2024 Barcelona
- Pixelgen:
- Pixelgen Technologies homepage
- @PixelgenTech on twitter / X and LinkedIn
- BioLizard:
- BioLizard homepage
- @BioLizard_nv on twitter / X and LinkedIn
Chapters:
- Start (00:00)
- Welcome (00:09)
- Introductions (00:50)
- Intro to Pixelgen Technologies (01:41)
- Lab methods (03:08)
- Nature Methods publication (06:15)
- Data visualisation (09:35)
- Working with single cell data (12:29)
- Cell interactions (15:06)
- Why Pixelgen chose open source (16:05)
- Pixelator Python package (20:10)
- Building within nf-core (21:40)
- Florian - Biolizard (23:01)
- Pipeline overview (25:19)
- Using nf-core from a commercial setting (26:50)
- Coming out of stealth (28:53)
- Extending Pixelator (32:24)
- Dealing with customers (34:58)
- Looking to the future (39:38)
- Interactive analysis (41:32)
- Advice for others (43:12)
- Pixelgen at the Nextflow Summit (45:16)
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